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Dive Deep into the World of Molecular Biology
Molecular biology, a fascinating branch of biology, delves into the intricate world of biological macromolecules like nucleic acids and proteins, unraveling their roles in the replication of cells and the transmission of genetic information. This field is not just about understanding the complex biological processes; it also involves hands-on manipulation of DNA to explore the vast genetic landscapes and their implications in various biological phenomena.
Comprehensive Molecular Biology Course Topics
Embarking on a molecular biology course, students will navigate through an extensive array of topics, each shedding light on different facets of cellular and molecular functions. These topics include, but are not limited to:
- Cells and genomes
- Cell chemistry and biosynthesis
- The structure and function of proteins
- DNA, chromosomes, and their intricate dynamics
- Mechanisms of DNA replication, repair, and recombination
- Gene expression and its regulation
- Techniques for manipulating proteins, DNA, and RNA
- Advanced visualization methods for studying cells
- Membrane structure and function, including transport mechanisms
- Intracellular compartments, vesicular traffic, and protein sorting
- The fundamentals of energy conversion in mitochondria and chloroplasts
- Cell communication, the cytoskeleton, cell cycle, and apoptosis
- The mechanics of cell division and the molecular basis of cancer
- The immune system and its responses to pathogens
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Recently Asked Molecular Biology Questions
Expert help when you need it- Q1:Write a one paragraph response explaining the four different perspectives below on human cloning. Introduce the authors’ full names (titles aren’t necessary for this paragraph, but they would be for an essay) to indicate each of their different opinions. Consider the most strategic way of how to organize/structure your paragraph to show connections and contrasts between each of the source’s arguments Use transitions carefully, as they will show how you relate the articles to each other. Perspective 1: Patricia Baird, “Should Human Cloning be Permitted?” “...In conclusion, using nuclear-transfer cloning to allow people to have a child introduces a different way of reproduction for our species. Once we breach this barrier, it leaves us with no place to stop. Given all the problems outlined, the reasons for permitting cloning to produce a person are insufficiently compelling. Even in the few circumstances where the case for human cloning seems justified, there are alternative solutions. We are at an appropriate stopping place on a slippery slope. Not all reasons why a person might wish to copy his or her cells are unethical, but given there are other options open to people wishing to form a family, concerns about individual and social harms from cloning are strong enough that it is not justified to permit it.” Perspective 2: Chris MacDonald, “Yes, Human Cloning Should be Permitted.” “The fact that a portion of society—even a majority—finds an activity distasteful is insufficient grounds for passing a law forbidding it....human cloning for reproductive purposes has legitimate, morally acceptable applications—for example for infertile couples, and for gay couples.” Perspective 3: Jacob M. Appel, “Should We Really Fear Reproductive Human Cloning?” “In an ideal world, human reproductive cloning would be safe, legal and rare. I say rare because my guess is that the vast majority of people, myself included, would have little desire to raise cloned offspring. After all, it is now possible to clone pet dogs--but few of us would choose to spend a spare $150,000 on such a venture. Yet thirty-eight years after James Watson's seminal essay, "Moving Toward the Clonal Man" called for increased public debate on this promising and perplexing subject, I don't believe that we should be so quick to greet cloning technology with a permanent injunction. Instead, what human reproductive cloning requires at the moment is a yellow light, telling us to proceed with extreme caution, until we know with confidence whether the technology can ever be used to produce healthy babies.” Perspective 4: Leon Kass, “The Wisdom of Repugnance” “We are repelled by the prospect of cloning human beings not because of the strangeness or novelty of the undertaking, but because we intuit and feel, immediately and without argument, the violation of things that we rightfully hold dear. Repugnance, here as elsewhere, revolts against the excesses of human wilfulness, warning us not to transgress what is unspeakably profound. Indeed, in this age in which everything is held to be permissible so long as it is freely done, in which our given human nature no longer commands respect, in which our bodies are regarded as mere instruments of our autonomous rational wills, repugnance may be the only voice left that speaks up to defend the central core of our humanity.” See Answer
- Q2:b. C. d. What was the independent variable in this experiment? What was the dependent variable? What were the standardized variables?See Answer
- Q3:e. f. g. Do the results support your hypothesis? Explain. At which temperature was the rate of osmosis greatest? What can you conclude about the effects of temperature on the rate of osmosis? Answer in terms of molecular energy.See Answer
- Q4:h. Name at least two possible sources of error in this experiment. How could this experiment be improved?See Answer
- Q5:Variables Table 1 - Osmosis: Effect of Temperature Cold Beaker Water temperature Tube mass Tube firmness Warm Beaker Room Temp Beaker Water temperature Tube mass Tube firmness Before After % Change Observations Water temperature Tube mass Tube firmnessSee Answer
- Q6:Variable Water Temperature Tube Mass Variable Water Temperature Tube Mass Before Tube Mass Table 1 B Room Temperature Table 1B Before weight in grams of pseudocell containing 40% sucrose, and weight after 40 minutes incubation n water at room temperature in degrees Celsius. After % Change in weight Before 102, POLAR W 10.7 After ACCULAB 109 11.6 % Change in weight 9 Table 1 C Warm Beaker Table 1C Before weight in grams of pseudocell containing 40% sucrose, and weight after 40 minutes incubation in warm water. Variable Before After % Change in weight Observations Water Temperature 16.6 Observations ObservationsSee Answer
- Q7:HANDS-ON ACTIVITY#1: 1. Get 2 transparent glasses (same size) 2. Put the same volume of water in both of them. In one of the glasses put cold water and in the other glass put hot water. 3. Place the 2 glasses side by side and let the water sit for a few seconds. 4. Put a drop of food dye in each of the glasses. Use your cell phone to take a picture of this hands-on activity. You will need to submit this picture and your photo ID to DB 2. Answer the following questions: Why does 1 drop of the dye stain the entire volume of water? Does the staining of the entire volume of water happen faster/slower/or at the same speed in the hot water compared to the cold water? Why? HANDS-ON ACTIVITY#2: This experiment will take you 3 days to complete. Perform the experiment and answer questions 1 to 7 in this hand-out. Use your cell phone to take pictures of this hands-on activity. You will need to submit these pictures and your photo ID to DB 2. An unfertilized chicken egg contains a large cell surrounded by egg white, a shell membrane, and an egg shell. You will investigate how the size of an egg changes when the eggshell is removed and the egg is placed in different types of liquid. ➤ Get 2 eggs. To begin, record the weight or circumference of each egg in the day 1 row in the table. (Measure the circumference around the widest part, not lengthwise.) Day 1 2 Egg 1 Egg 2 Weight (grams) (or circumference (cm)) Weight (grams) (or circumference (cm)) (with shell) (with shell) (after a day in vinegar most of shell removed) (after a day in vinegar: most of shell removed)See Answer
- Q8:3 (after a day in water) (after a day in corn syrup) Put each egg in a container labeled Egg 1 or Egg 2. Pour in enough vinegar to cover the egg. Cover the container. Do you see bubbles forming around the egg? These are bubbles of CO2 which result from the chemical reaction between the acetic acid in the vinegar and the calcium carbonate in the eggshell. This reaction will dissolve most of the eggshell by day 2. Day 2 ➤ Observe your eggs. Notice that most of the shell has been dissolved by the acetic acid in the vinegar. The shell membrane around the egg is fairly strong. However, the egg without its shell is fragile, so you will need to handle your eggs very gently and carefully! Rinse each egg and measure the weight or circumference of each egg. Record your results for day 2 in the above table. 1a. Did the eggs become heavier/larger or lighter/smaller 1b. What do you think happened to cause the change in the eggs' weight/size? ➤ Empty the vinegar from the container for egg 1 and rinse the container. Put egg 1 back in the container and add water to cover the egg. ➤ Empty the vinegar from the container for egg 2 and rinse the container. Put egg 2 back in the container and add corn syrup to cover the egg. Day 3 2. Compare and contrast the appearance of the egg that has been in water vs. the egg that has been in corn syrup. 3. You may be able to see a layer of water on top of the corn syrup. Where do you think this water came from? Rinse the corn syrup off of egg 2. Measure and record the weight and/or circumference of the egg for day 3 in the table on page 1. 4. Why did the egg placed in water get heavier and bigger? Where do you think the additional weight/volume came from?See Answer
- Q9:5. What do you think happened to cause the change in weight/size of the egg placed in corn syrup? 6a. Recall that each egg is surrounded by a shell membrane. Based on your observations, which of the following do you think can cross this membrane? a. both water and the proteins in the egg white b. water, but not the proteins in the egg white c. the proteins in the egg white, but not water d. neither water nor the proteins in the egg white 6b. What evidence supports your conclusion? 7. The shell membrane that surrounds the egg is a selectively permeable membrane. Explain why "selectively permeable" is a good way to describe this membrane.See Answer
- Q10:Use the article "Fundamental mechanisms of telomerase action in yeasts and mammals: understanding telomeres and telomerase in cancer cells" (below) to answer the following questions https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376709/ When reading the research article, read the abstract, section 1.1, 1.2, Figure 1, Figure 2, 3.1, 3.2, 3.4 and 4.1. Questions: 1. Based on what you read, what is telomerase and why is it important? (3 points) 2. Based on what you read, is telomerase evolutionarily conserved? Provide evidence from the article to support your answer (3 points)See Answer
- Q11:Inflammation is one of the crucial processes of innate immunity that alerts the immune system and recruits the needed cells, clears the damaged area, and then sets the stage for subsequent tissue repair or regeneration. Despite the importance of inflammation to immunity and the role of chronic inflammation in many common pathologies, we have only begun to gain a basic understanding of the major events that initiate, regulate, and inhibit the process. Although we have known that neutrophils are among the first cells to arrive on the scene of an inflammatory response, we have only recently begun to understand the important role that these cells play in the process. It was discovered in the mid-2000s that neutrophils form structures dubbed neutrophil extracellular traps, or NETS, when activated in an inflammatory response. Briefly, what are NETS, what is in them, and what purpose do they serve in inflammation and immunity? What are a few of the major pathologies that NETs have been implicated in?See Answer
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